- For correlative cryo-light (upright) and cryo-electron microscopy (CLEM)
- -190°C - 120°C with resistive heating and liquid nitrogen cooling option
- Grid holder allows nine EM grids, 3 mm round, to be imaged via bright field and fluorescence light microscopy to improve productivity
- Grid holder designed for vitreous operation and transfer far below the amorphous ice phase transition temperature
Description
Instec's CLM77K is a specialized stage designed for use on any upright microscope for correlative cryo-light and cryo-electron microscopy (CLEM). The specially designed grid holder allows up to nine 3 mm EM grids to be images via bright field and flourescence light microscopy for determination of regions and/or cellular events of interest for later observation in the electron microscope. The grid holder is designed to keep the grids vitreous - well below the amorphous ice phase transition temperature - during tranfer of grids into the grid holder and then into the CLM77K stage. To eliminate frost build-up on samples, the chamber can be gas purged and the grid holder has an integrated slide cover that is closed during transfer from liquid nitrogen into the CLM77K chamber.
Thermal Features
|
Temperature Control |
mK2000 with programmable precision switching PID method |
|
Thermal Block |
Black anodized aluminum body |
|
Sample Thermal Cover |
Integrated slide cover on grid holder to eliminate moisture condensation on samples |
|
Temperature Minimum |
-190°C (with optional liquid N2 cooling) |
|
Temperature Maximum |
120°C |
|
Temperature Sensor |
100 Ω Platinum RTD |
|
Maximum Heating Rate |
+60°C per minute at 100°C |
|
Maximum Cooling Rate |
-50°C per minute at 100°C |
|
Minimum Heating and Cooling Rate |
±0.01°C per minute |
|
Temperature Resolution |
0.01°C |
|
Temperature Stability |
±0.05°C (above 25°C), ±0.1°C (below 25°C) |
|
Power Supply |
Universal power input |
|
Software |
Windows software to record and export temperature-time data |
Optical Features
|
Optical Access |
Reflection and transmission capability |
|
Optical Windows |
Removable and exchangeable windows permit full-spectrum transparency |
|
Minimum Objective Working Distance |
5.0 mm |
|
Minimum Condenser Working Distance |
17.6 mm |
|
Top Window Aperture |
46 mm diameter |
|
Top Viewing Angle |
±45.73° from normal |
|
Transmission Aperture |
2 mm diameter（9x） |
|
Bottom Window Aperture |
19 mm diameter |
|
Bottom Viewing Angle |
±9.95° from normal |
|
Window Defrost |
Integrated external window defrost |
Structure Features
|
Sample Area |
Holds 9 grids of 3mm in diameter |
|
Grid Height |
0.25 mm |
|
Sample Loading |
Quick sample handling with side loading/unloading holder |
|
Frame Cooling |
Integrated frame cooling with optional chiller system |
|
Mounting |
Horizontal mounting capability |
|
Frame Dimensions |
218.8 mm x 116.8 mm x 24.5 mm |
|
Weight |
1500 g |
